Powerful tool for the physiological formation of 3D human induced pluripotent stem cell (hiPSC) spheroids in vitro. hiPSC spheroids maintain high viability, pluripotency and maintain differentiation potential based on multiple biomarkers.
- Kit consists of a vial of Synthegel 3D hiPSC peptide nanofiber solution and a vial of Synthegel X-Link solution
- Requires a vial of lyophilized Synthegel 3D hiPSC Grow Mix
- Matrix rigidity is tunable by altering the peptide concentration
- Purified synthetic peptide in neutral pH
- Cells are encapsulated in physiological mimicking conditions
- Fast hydrogel formation (5 to 30 minutes)
- Sol-gel transfer reversible by shearing force
- Works with standard cell culture methodologies, growth factors and media
- Allows growth and passaging of hiPSCs in 3D format
- The hiPSC Grow Mix supplement provides a complete environment for hiPSC 3D culture
- Allows 3D embedded hiPSC cultures
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